{"id":7046321,"date":"2026-08-24T17:15:33","date_gmt":"2026-08-24T17:15:33","guid":{"rendered":"https:\/\/peraltafinancing.com\/uncategorized\/anticonvulsant-effects-safety-and-sex-dependent-molecular-signatures-of-cannabidiol-treatment-in-a-preclinical-model-of-audiogenic-epilepsy\/"},"modified":"2026-08-24T17:15:33","modified_gmt":"2026-08-24T17:15:33","slug":"anticonvulsant-effects-safety-and-sex-dependent-molecular-signatures-of-cannabidiol-treatment-in-a-preclinical-model-of-audiogenic-epilepsy","status":"publish","type":"post","link":"https:\/\/fivemor.com\/?p=7046321","title":{"rendered":"Anticonvulsant effects, safety, and sex-dependent molecular signatures of cannabidiol treatment in a preclinical model of audiogenic epilepsy"},"content":{"rendered":"<p> <br \/>\n<\/p>\n<div id=\"eng-abstract\">\n<p>      Roughly one-third of patients with epilepsy remain drug resistant, underscoring the need for novel therapeutic strategies. Although cannabidiol (CBD) has recently been approved for specific epileptic encephalopathies, its anticonvulsant mechanisms and the influence of biological sex on treatment response remain incompletely understood. In this preclinical study, we evaluated the efficacy, tolerability, pharmacokinetics, and molecular effects of CBD in the GASH\/Sal hamster, a genetic model of audiogenic generalized tonic-clonic seizures. Animals received intraperitoneal CBD (200 mg\/kg) either acutely or chronically for 14 days. CBD concentrations were measured in serum and brain, while seizure severity, latency, and neuroethological parameters were assessed following acoustic seizure induction. Safety was evaluated through body weight, hematological, and biochemical analyses, and gene expression profiling was performed in the inferior colliculus, the primary epileptogenic focus. CBD achieved measurable systemic and brain exposure after both acute and chronic administration, despite substantial inter-individual variability and no significant sex differences in drug concentrations. CBD reduced audiogenic seizure severity in a time-dependent manner, with greater protection after chronic treatment. CBD also prolonged latency to seizure onset in both sexes (earlier in females). Although complete seizure suppression was more frequent in females than males (37.5% vs. 12.5% after chronic treatment), direct sex comparisons did not reach statistical significance. Notably, higher serum and brain CBD concentrations were associated with lower seizure severity. Chronic CBD administration was well tolerated in both sexes, affecting selected hematological parameters without altering body weight or liver function. Gene expression profiling revealed that transcriptional organization of the inferior colliculus was driven predominantly by biological sex rather than by seizure induction or CBD treatment. Marked sex-dependent differences were observed in serotonergic, endocannabinoid, purinergic, and Sigmar1-related neuroprotective pathways. Within this molecular context, seizure stimulation modulated <i>Trpv1<\/i> and <i>Slc29a1<\/i>, whereas chronic CBD induced pathway-specific, sex-dependent changes involving <i>5-Htr1a<\/i>, <i>Adora1<\/i>, and <i>Cnr1<\/i>, without eliciting widespread transcriptional remodeling. Collectively, these findings identify CBD as a well-tolerated anticonvulsant in the GASH\/Sal model and suggest that pharmacokinetic exposure and the sex-specific molecular organization of the epileptogenic focus contribute to variability in treatment response, highlighting the importance of considering biological sex in cannabinoid-based epilepsy research.\n    <\/p>\n<\/p><\/div>\n<p>        <strong class=\"sub-title\"><br \/>\n          Keywords:<br \/>\n        <\/strong><\/p>\n<p>      animal models; anticonvulsant therapy; cannabis; epilepsy; gene expression; neuroethology; pharmacokinetics; sex differences.\n    <\/p>\n\n","protected":false},"excerpt":{"rendered":"<p>Roughly one-third of patients with epilepsy remain drug resistant, underscoring the need for novel therapeutic strategies. Although cannabidiol (CBD) has recently been approved for specific epileptic encephalopathies, its anticonvulsant mechanisms and the influence of biological sex on treatment response remain incompletely understood. In this preclinical study, we evaluated the efficacy, tolerability, pharmacokinetics, and molecular effects [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":353883,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[135601],"tags":[208775,208778,125019,208771,208773,6228,86408,208772,175060,1168,81772,175058,56813,175059,175057,41397,175056,208770,208769,208777,175055,208774,417,208776,71900,14368],"dealstore":[],"offerexpiration":[],"class_list":["post-7046321","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hemp","tag-anticonvulsant","tag-audiogenic","tag-cannabidiol","tag-doi10-3389-fphar-2026-1745696","tag-dolores-e-lopez","tag-effects","tag-epilepsy","tag-giselda-cabral-pereira","tag-medline","tag-model","tag-molecular","tag-national-center-for-biotechnology-information","tag-national-institutes-of-health","tag-national-library-of-medicine","tag-ncbi","tag-nih","tag-nlm","tag-pmc13493383","tag-pmid42630256","tag-preclinical","tag-pubmed-abstract","tag-ricardo-gomez-nieto","tag-safety","tag-sexdependent","tag-signatures","tag-treatment"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Anticonvulsant effects, safety, and sex-dependent molecular signatures of cannabidiol treatment in a preclinical model of audiogenic epilepsy - Som2ny Network<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fivemor.com\/?p=7046321\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Anticonvulsant effects, safety, and sex-dependent molecular signatures of cannabidiol treatment in a preclinical model of audiogenic epilepsy - Som2ny Network\" \/>\n<meta property=\"og:description\" content=\"Roughly one-third of patients with epilepsy remain drug resistant, underscoring the need for novel therapeutic strategies. Although cannabidiol (CBD) has recently been approved for specific epileptic encephalopathies, its anticonvulsant mechanisms and the influence of biological sex on treatment response remain incompletely understood. In this preclinical study, we evaluated the efficacy, tolerability, pharmacokinetics, and molecular effects [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/fivemor.com\/?p=7046321\" \/>\n<meta property=\"og:site_name\" content=\"Som2ny Network\" \/>\n<meta property=\"article:published_time\" content=\"2026-08-24T17:15:33+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/fivemor.com\/wp-content\/uploads\/2026\/07\/pubmed-meta-image-v2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1600\" \/>\n\t<meta property=\"og:image:height\" content=\"830\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/fivemor.com\/?p=7046321#article\",\"isPartOf\":{\"@id\":\"https:\/\/fivemor.com\/?p=7046321\"},\"author\":{\"name\":\"admin\",\"@id\":\"https:\/\/fivemor.com\/#\/schema\/person\/b85e3c3dc0e1daea076524dc8810c371\"},\"headline\":\"Anticonvulsant effects, safety, and sex-dependent molecular signatures of cannabidiol treatment in a preclinical model of audiogenic epilepsy\",\"datePublished\":\"2026-08-24T17:15:33+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/fivemor.com\/?p=7046321\"},\"wordCount\":381,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/fivemor.com\/#organization\"},\"image\":{\"@id\":\"https:\/\/fivemor.com\/?p=7046321#primaryimage\"},\"thumbnailUrl\":\"https:\/\/fivemor.com\/wp-content\/uploads\/2026\/07\/pubmed-meta-image-v2.jpg\",\"keywords\":[\"Anticonvulsant\",\"audiogenic\",\"Cannabidiol\",\"doi:10.3389\/fphar.2026.1745696\",\"Dolores E L\u00f3pez\",\"Effects\",\"Epilepsy\",\"Giselda Cabral-Pereira\",\"MEDLINE\",\"Model\",\"Molecular\",\"National Center for Biotechnology Information\",\"National Institutes of Health\",\"National Library of Medicine\",\"NCBI\",\"NIH\",\"NLM\",\"PMC13493383\",\"pmid:42630256\",\"preclinical\",\"PubMed Abstract\",\"Ricardo G\u00f3mez-Nieto\",\"Safety\",\"sexdependent\",\"Signatures\",\"Treatment\"],\"articleSection\":[\"Hemp\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/fivemor.com\/?p=7046321#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/fivemor.com\/?p=7046321\",\"url\":\"https:\/\/fivemor.com\/?p=7046321\",\"name\":\"Anticonvulsant effects, safety, and sex-dependent molecular signatures of cannabidiol treatment in a preclinical model of audiogenic epilepsy - 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