{"id":45613,"date":"2025-01-24T21:07:32","date_gmt":"2025-01-24T21:07:32","guid":{"rendered":"https:\/\/peraltafinancing.com\/aquarium\/hydrospace-releases-deep-cycle-a-consortium-of-real-reef-microbes-reef-builders\/"},"modified":"2025-01-24T21:07:32","modified_gmt":"2025-01-24T21:07:32","slug":"hydrospace-releases-deep-cycle-a-consortium-of-real-reef-microbes-reef-builders","status":"publish","type":"post","link":"https:\/\/fivemor.com\/?p=45613","title":{"rendered":"Hydrospace Releases \u201cDeep Cycle\u201d A Consortium of Real Reef Microbes | Reef Builders"},"content":{"rendered":"<p> <br \/>\n<\/p>\n<div>\n<h2 class=\"wp-block-heading\">Intensive R&amp;D and Transparency<\/h2>\n<p id=\"viewer-mu75l55\">In 2022 Hydrospace went on an exhibition to French Polynesia where they collected sediment from a real reef. Since then Kenneth the owner of Hydrospace has been hard at work cultivating the microbes found on that sand. After a few years of curation a stable microbial community has emerged from the samples. Instead of a monoculture (one species) a functional guild or polyculture (multiple species living together in symbiosis) has emerged.<\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/static.wixstatic.com\/media\/21dddd_3905949c9dff44169e1b231445d31198~mv2.jpg\/v1\/fill\/w_620,h_465,al_c,q_80,usm_0.66_1.00_0.01,enc_auto\/21dddd_3905949c9dff44169e1b231445d31198~mv2.jpg\" alt=\"\"\/><\/figure>\n<p id=\"viewer-ay4h365\">Collection site in French Polynesia<\/p>\n<p>The polyculture consist of the following species per Hydrospace (confirmed with DNA sequencing)<\/p>\n<p>\u201c<em>Rhodovolum<\/em>\u00a0sp. (purple nonsulfur bacteria), <em>Marichromatium purpuratum<\/em>\u00a0(purple sulfur bacteria) and <em>Prosthecochloris<\/em>\u00a0sp. (green sulfur bacteria). <em>Roseospira<\/em>\u00a0sp. (purple nonsulfur bacteria) makes a smaller, but nevertheless significant, appearance as well. At maturity, these cultures present as a golden green suspension (primarily <em>Rhodovolum<\/em> and <em>Prosthecochloris<\/em>).\u201d<\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/static.wixstatic.com\/media\/21dddd_9ea0a6b4d0434f50958057afa4ab6da9~mv2.jpg\/v1\/fill\/w_620,h_672,al_c,q_85,usm_0.66_1.00_0.01,enc_auto\/21dddd_9ea0a6b4d0434f50958057afa4ab6da9~mv2.jpg\" alt=\"\"\/><\/figure>\n<p id=\"viewer-zojj073\">The initial culture<\/p>\n<h2 class=\"wp-block-heading\">Product Function<\/h2>\n<p>This polyculture is what goes into a bottle of Hydrospace Deep Cycle. Per Hydrospace the species have the following functions and benefits in marine aquaria:<\/p>\n<ul class=\"wp-block-list\">\n<li>\u201c<em>Rhodovolum<\/em>\u00a0sp. improves water quality through the consumption of dissolved organic matter (<a href=\"https:\/\/academic.oup.com\/lambio\/article-abstract\/38\/1\/13\/6703259?redirectedFrom=fulltext\" target=\"_blank\" rel=\"noreferrer noopener\">1<\/a>), whereafter it becomes a nutritious live food due in part to its probiotic properties (<a href=\"https:\/\/www.mdpi.com\/2076-2607\/10\/2\/244\" target=\"_blank\" rel=\"noreferrer noopener\"><u>2<\/u><\/a>, <a href=\"https:\/\/www.siftdesk.org\/article-details\/The-effects-of-a-marine-photosynthetic-bacteria-emRhodovulum-sulfidophilumem-on-the-growth-and-survival-rate-of-emMarsupenaeus-japonicusem-kuruma-shrimp\/10713\" target=\"_blank\" rel=\"noreferrer noopener\"><u>3<\/u><\/a>, <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S1878818118300252\" target=\"_blank\" rel=\"noreferrer noopener\"><u>4<\/u><\/a>) and relatively high content of fatty acids (<a href=\"http:\/\/borneoscience.ums.edu.my\/wp-content\/uploads\/2012\/03\/SUITABILITY-OF-SEAWEED-MEAL-INCORPORATED-WITH-RHODOVULUM-SP.-BACTERIUM-AS-FEED-SUPPLEMENT-FOR-FINFISH-LARVAE1.pdf\" target=\"_blank\" rel=\"noreferrer noopener\"><u>5<\/u><\/a>, <a href=\"https:\/\/www.researchgate.net\/publication\/337676702_IDENTIFICATION_AND_CHARACTERIZATION_OF_A_PURPLE_NONSULFUR_BACTERIUM_ISOLATED_FROM_COASTAL_AREA_OF_HAI_PHONG_FOR_USING_IN_PRODUCTION_OF_UNSATURATED_FATTY_ACID_OMEGA_6_7_9\" target=\"_blank\" rel=\"noreferrer noopener\"><u>6<\/u><\/a>, <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1111\/j.1365-2109.2012.03193.x\" target=\"_blank\" rel=\"noreferrer noopener\"><u>7<\/u><\/a>, <a href=\"https:\/\/core.ac.uk\/download\/pdf\/270210522.pdf\" target=\"_blank\" rel=\"noreferrer noopener\"><u>8<\/u><\/a>).\u00a0<\/li>\n<li><em>Marichromatium purpuratum<\/em>\u00a0improves water quality by consuming dissolved organic matter, reducing nitrate and sulfide concentrations (<a href=\"https:\/\/www.nature.com\/articles\/s41598-018-36160-2\" target=\"_blank\" rel=\"noreferrer noopener\"><u>9<\/u><\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25857753\/\" target=\"_blank\" rel=\"noreferrer noopener\"><u>10<\/u><\/a>, <a href=\"https:\/\/academic.oup.com\/femsle\/article-abstract\/180\/2\/317\/527833?redirectedFrom=fulltext\" target=\"_blank\" rel=\"noreferrer noopener\"><u>11<\/u><\/a>) and providing probiotic benefits (<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0044848617307883\" target=\"_blank\" rel=\"noreferrer noopener\"><u>12<\/u><\/a>).<\/li>\n<li><em>Prosthecochloris<\/em>\u00a0sp. plays important roles in nitrogen and sulfur cycling (<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19436133\/\" target=\"_blank\" rel=\"noreferrer noopener\"><u>13<\/u><\/a>, <a href=\"https:\/\/www.frontiersin.org\/journals\/microbiology\/articles\/10.3389\/fmicb.2023.1138751\/full\" target=\"_blank\" rel=\"noreferrer noopener\"><u>14<\/u><\/a>, <a href=\"https:\/\/aslopubs.onlinelibrary.wiley.com\/doi\/10.1002\/lno.10277\" target=\"_blank\" rel=\"noreferrer noopener\"><u>15<\/u><\/a>, <a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/9780470015902.a0029145\" target=\"_blank\" rel=\"noreferrer noopener\"><u>16<\/u><\/a>) and likely forms a symbiotic relationship with stony corals as an endolithic diazotroph (<a href=\"https:\/\/www.frontiersin.org\/journals\/marine-science\/articles\/10.3389\/fmars.2023.1055848\/full\" target=\"_blank\" rel=\"noreferrer noopener\"><u>17<\/u><\/a>, <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1470160X24000840\" target=\"_blank\" rel=\"noreferrer noopener\"><u>18<\/u><\/a>).\u201d<\/li>\n<\/ul>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/static.wixstatic.com\/media\/21dddd_a16362d484ef410984d51cfd6dc03735~mv2.png\/v1\/fill\/w_620,h_362,al_c,q_85,usm_0.66_1.00_0.01,enc_auto\/21dddd_a16362d484ef410984d51cfd6dc03735~mv2.png\" alt=\"\"\/><\/figure>\n<p>Image of the Aquabiomics data<\/p>\n<p>Adding these species to an aquarium likely assists with nutrient cycling in the substrate and in the corals themselves since <em>Prosthecochloris<\/em> are capable of forming symbiotic relationships with corals and colonizing their skeletons. Overall, these species likely benefit the microbial balance of a system and are representative of species not found in any other product on the market.<\/p>\n<h2 class=\"wp-block-heading\">How Do I Use It?<\/h2>\n<p>Below are instructions of use from Hydrospace<\/p>\n<p id=\"viewer-zkoa7141\">\u201cPNS Deep Cycle\u2122 contains several obligately anaerobic species; therefore, protecting the cultures from oxygen exposure may promote successful colonization of the substrate. One simple means of carrying out an oxygen-free transfer is by injection. Any syringe with a long needle or tube is suitable for this purpose. Slowly inject the pre-measured dose as deeply, and across as many injection sites, as possible into the substrate.<\/p>\n<p id=\"viewer-4gaxy10653\">Similarly, direct transfer to dry rock and porous biomedia can promote colonization of these bacteria. This is accomplished simply by soaking the material in the product under anaerobic conditions (i.e.<a href=\"http:\/\/i.e.no\/\" target=\"_blank\" rel=\"noreferrer noopener\">, no<\/a> aeration). Place the rock\/media in a clean, sealed container that is just large enough to contain the material. Add to the container a sufficient volume of PNS Deep Cycle\u2122 to thoroughly soak the material. Add a sufficient volume of clean saltwater to displace any remaining air to the rim of the container. Place lid on the container and allow material to condition for 24 hours. After the conditioning period, the material may be added (along with the water in the container) to the tank, filter system, etc.<\/p>\n<p>Store at room temperature. Treats 400 gallons. Disperse 1.25 mL\/gal broadly into an area of moderate flow; may also be injected or soaked into substrate or biomedia for maximal efficacy (scan QR code to review instructions for special applications); multiple doses can be safely applied and may increase likelihood of permanent colonization. For optimal results,\u00a0<em>cut protein skimmer, UV sterilizer and ozone generator<\/em>\u00a0for one hour following each dose.\u201d<\/p>\n<p><a href=\"https:\/\/aquariumsupplydistribution.com\/\">Aquarium Supply and Distribution<\/a> is a distributor of Hydrospace products \u2013 if you are interested in this product please reach out to them or have your LFS do so on your behalf.<\/p>\n<p>For more information on this product view our video where we tour Ken\u2019s facility and interview him on the new product <a href=\"https:\/\/www.youtube.com\/watch?v=LHwayirMgL0\">here<\/a><\/p>\n<p>You can also check out a more in depth explanation of these species roles <a href=\"https:\/\/www.microbiofarm.com\/post\/strength-in-diversity-syntrophic-relations-between-anaerobic-photosynthetic-bacteria?fbclid=IwY2xjawIAp6hleHRuA2FlbQIxMQABHTd6Pc2b9uIAj-E_Q9B7bQZ2TRaCeqTDULk5BKmYeyiSZi28a4csKT_LNQ_aem_apekhj-K395znR28n1gYhw\">here<\/a><\/p>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Intensive R&amp;D and Transparency In 2022 Hydrospace went on an exhibition to French Polynesia where they collected sediment from a real reef. Since then Kenneth the owner of Hydrospace has been hard at work cultivating the microbes found on that sand. After a few years of curation a stable microbial community has emerged from the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":45614,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12040],"tags":[10323,27805,3346,1636,27804,27806,482,14531,3937],"dealstore":[],"offerexpiration":[],"class_list":["post-45613","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-aquarium","tag-builders","tag-consortium","tag-cycle","tag-deep","tag-hydrospace","tag-microbes","tag-real","tag-reef","tag-releases"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Hydrospace Releases \u201cDeep Cycle\u201d A Consortium of Real Reef Microbes | Reef Builders - Som2ny Network<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fivemor.com\/?p=45613\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Hydrospace Releases \u201cDeep Cycle\u201d A Consortium of Real Reef Microbes | Reef Builders - Som2ny Network\" \/>\n<meta property=\"og:description\" content=\"Intensive R&amp;D and Transparency In 2022 Hydrospace went on an exhibition to French Polynesia where they collected sediment from a real reef. 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